Proteintech, 16244-1-AP, ATP13A1 Polyclonal antibody

Size: 20ul / 150ul The ATP13A1 (16244-1-AP) by Proteintech is a Polyclonal antibody targeting ATP13A1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 16244-1-AP targets ATP13A1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse liver tissue, rat liver tissue Positive IP detected in: HeLa cells Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information ATP13A1 is a P-type ATPase, mainly involved in the transport of cations within cells. It plays an important role in maintaining intracellular ion homeostasis and membrane potential. ATP13A1 acts as a dislocase that mediates the ATP-dependent extraction of mislocalized mitochondrial transmembrane proteins from the endoplasmic reticulum membrane (PubMed:32973005). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9174 Product name: Recombinant human ATP13A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 615-758 aa of BC009302 Sequence: QKEFVITSLKELGYVTLMCGDGTNDVGALKHADVGVALLANAPERVVERRRRPRDSPTLSNSGIRATSRTAKQRSGLPPSEEQPTSQRDRLSQVLRDLEDESTPIVKLGDASIAAPFTSKLSSIQCICHVIKQGRCTLVTTLQM Predict reactive species Full Name: ATPase type 13A1 Calculated Molecular Weight: 976 aa, 109 kDa Observed Molecular Weight: 120-130 kDa GenBank Accession Number: BC009302 Gene Symbol: ATP13A1 Gene ID (NCBI): 57130 RRID: AB_2290293 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9HD20 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.