Proteintech, 16257-1-AP, GGCT Polyclonal antibody

Size: 20ul / 150ul The GGCT (16257-1-AP) by Proteintech is a Polyclonal antibody targeting GGCT in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 16257-1-AP targets GGCT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, U-937 cells, mouse kidney tissue, mouse liver tissue, human brain tissue, HepG2 cells, HEK-293 cells, Jurkat cells Positive IP detected in: mouse kidney tissue Positive IHC detected in: mouse small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9284 Product name: Recombinant human GGCT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-188 aa of BC019243 Sequence: MANSGCKDVTGPDEESFLYFAYGSNLLTERIHLRNPSAAFFCVARLQDFKLDFGNSQGKTSQTWHGGIATIFQSPGDEVWGVVWKMNKSNLNSLDEQEGVKSGMYVVIEVKVATQEGKEITCRSYLMTNYESAPPSPQYKKIICMGAKENGLPLEYQEKLKAIEPNDYTGKVSEEIEDIIKKGETQTL Predict reactive species Full Name: gamma-glutamyl cyclotransferase Calculated Molecular Weight: 188 aa, 21 kDa Observed Molecular Weight: 21-25 kDa GenBank Accession Number: BC019243 Gene Symbol: GGCT Gene ID (NCBI): 79017 RRID: AB_10852962 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75223 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.