Proteintech, 16270-1-AP, PPCDC Polyclonal antibody

Size: 20ul / 150ul The PPCDC (16270-1-AP) by Proteintech is a Polyclonal antibody targeting PPCDC in WB, ELISA applications with reactivity to human samples 16270-1-AP targets PPCDC in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, human placenta tissue, HL-60 cells, K-562 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC, one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine. The antibody was generated against full length human recombinant PPCDC, which is a bright yellow protein, indicating the presence of flavins. The antibody solution gives yellow color, too. It may be caused by elution of flavins during affinity purification with PPCDC-protein column. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9337 Product name: Recombinant human PPCDC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-204 aa of BC014409 Sequence: MEPKASCPAAAPLMERKFHVLVGVTGSVAALKLPLLVSKLLDIPGLEVAVVTTERAKHFYSPQDIPVTLYSDADEWEMWKSRSDPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLLTCVMRAWDRSKPLLFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPCVAKKLVCGDEGLGAMAEVGTIVDKVKEVLFQHSGFQQS Predict reactive species Full Name: phosphopantothenoylcysteine decarboxylase Calculated Molecular Weight: 204 aa, 22 kDa Observed Molecular Weight: 22-25 kDa, 14 kDa GenBank Accession Number: BC014409 Gene Symbol: PPCDC Gene ID (NCBI): 60490 RRID: AB_2299841 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96CD2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.