Proteintech, 16272-1-AP, SYAP1 Polyclonal antibody

Size: 20ul / 150ul The SYAP1 (16272-1-AP) by Proteintech is a Polyclonal antibody targeting SYAP1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 16272-1-AP targets SYAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, SMMC-7721 cells, A431 cells, mouse lung tissue, HepG2 cells, L02 cells, mouse liver tissue, rat lung tissue Positive IHC detected in: human cervical cancer tissue, human small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information SYAP1(Synapse-associated protein 1) is a human homologue of the Drosophila SAP47 (synapse associated protein), which is recognized by a monoclonal antibody that selectively stain synaptic terminals.This protein is a 352 amino acid protein that is ubiquitously expressed in adult tissues. SYAP1 contains one BSD domain which is is an approximately 60 amino acid long protein domain named after the BTF2-like transcription factors, Synapse-associated proteins and DOS2-like proteins in which it is found. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9342 Product name: Recombinant human SYAP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC014657 Sequence: MFRGLSSWLGLQQPVAGGGQPNGDALPEQPSETVAESAEEELQQAGDQELLHQAKDFGNYLFNFASAATKKITESVAETAQTIKKSVEEGKIDGIIDKTIIGDFQKEQKKFVEEQHTKKSEAAVPPWVDTNDEETIQQQILALSADKRNFLRDPPAGVQFNFDFDQMYPVALVMLQEDELLSKMRFALVPKLVKEEVFWRNYFYRVSLIKQSAQLTALAAQQQAAGKEEKSNGREQDLPLAEAVRPKTPPVVIKSQLKTQEDEEEISTSPGVSEFVSDAFDACNLNQEDLRKEMEQLVLDKKQEETAVLEEDSADWEKELQQELQEYEVVTESEKRDENWDKEIEKMLQEEN Predict reactive species Full Name: synapse associated protein 1, SAP47 homolog (Drosophila) Calculated Molecular Weight: 352 aa, 40 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: BC014657 Gene Symbol: SYAP1 Gene ID (NCBI): 94056 RRID: AB_2878236 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96A49 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.