Proteintech, 16278-1-AP, CHMP6 Polyclonal antibody

Size: 20ul / 150ul The CHMP6 (16278-1-AP) by Proteintech is a Polyclonal antibody targeting CHMP6 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 16278-1-AP targets CHMP6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, A375 cells, A431 cells, Jurkat cells, U-937 cells Positive IP detected in: mouse testis tissue Positive IHC detected in: human stomach tissue, human colon cancerNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information CHMP6 (charged multivesicular body protein 6) is a protein that plays a significant role in the formation of multivesicular bodies (MVBs) within cells. It is a component of the endosomal sorting complex required for transport III (ESCRT-III), which is involved in the sorting and transport of proteins within the cell. CHMP6 has been identified as a novel prognostic biomarker in bladder cancer, where it enhances cell survival and migration by regulating the cell cycle. (PMID: 40630215) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, canine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9357 Product name: Recombinant human CHMP6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-201 aa of BC010108 Sequence: MGNLFGRKKQSRVTEQDKAILQLKQQRDKLRQYQKRIAQQLERERALARQLLRDGRKERAKLLLKKKRYQEQLLDRTENQISSLEAMVQSIEFTQIEMKVMEGLQFGNECLNKMHQVMSIEEVERILDETQEAVEYQRQIDELLAGSFTQEDEDAILEELSAITQEQIELPEVPSEPLPEKIPENVPVKARPRQAELVAAS Predict reactive species Full Name: chromatin modifying protein 6 Calculated Molecular Weight: 201 aa, 23 kDa Observed Molecular Weight: 28-30 kDa GenBank Accession Number: BC010108 Gene Symbol: CHMP6 Gene ID (NCBI): 79643 RRID: AB_2079498 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96FZ7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.