Proteintech, 16317-1-AP, GCAT Polyclonal antibody

Size: 20ul / 150ul The GCAT (16317-1-AP) by Proteintech is a Polyclonal antibody targeting GCAT in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 16317-1-AP targets GCAT in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse pancreas tissue, rat pancreas tissue Positive IHC detected in: human pancreas tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:10000-1:40000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information GCAT (Glycine acetyltransferase) is an enzyme involved in the metabolism of amino acids, specifically in the degradation of L-threonine to glycine. GCAT expression can be regulated epigenetically, and its downregulation has been linked to mitochondrial respiration defects, particularly in aging cells (PMID: 26000717). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9367 Product name: Recombinant human GCAT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-419 aa of BC014457 Sequence: MWPGNAWRAALFWVPRGRRAQSALAQLRGILEGELEGICGAGTWKSERVITSRQGPHIRVDGVSGGILNFCANNYLGLSSHPEVIQAGLQALEEFGAGLSSVRFICGTQSIHKNLEAKIARFHQREDAILYPSCYDANAGLFEALLTPEDAVLSDELNHASIIDGIRLCKAHKYRYRHLDMADLEAKLQEAQKHRLRLVATDGAFSMDGDIAPLQEICCLASRYGALVFMDECHATGFLGPTGRGTDELLGVMDQVTIINSTLGKALGGASGGYTTGPGPLVSLLRQRARPYLFSNSLPPAVVGCASKALDLLMGSNTIVQSMAAKTQRFRSKMEAAGFTISGASHPICPVMLGDARLASRMADDMLKRGIFVIGFSYPVVPKGKAWIRVQISAVHSEEDIDRCVEAFVEVGRLHGALP Predict reactive species Full Name: glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase) Calculated Molecular Weight: 419 aa, 45 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC014457 Gene Symbol: GCAT Gene ID (NCBI): 23464 RRID: AB_2263046 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75600 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.