Proteintech, 16343-1-AP, SLC3A1 Polyclonal antibody

Size: 20ul / 150ul The SLC3A1 (16343-1-AP) by Proteintech is a Polyclonal antibody targeting SLC3A1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 16343-1-AP targets SLC3A1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, mouse kidney tissue, rat kidney tissue Positive IP detected in: mouse kidney tissue Positive IHC detected in: human kidney tissue, human small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information SLC3A1(Neutral and basic amino acid transport protein rBAT) is also named as NBAT,D2h,RBAT. It is involved in the high-affinity, sodium-independent transport of cystine and neutral and dibasic amino acids (system B(0,+)-like activity).SLC3A1 can be degraded, at least in part, via the endoplasmic reticulum-associated degradation (ERAD) pathway(PMID:18332091). SLC3A1 has different isoforms vary from 79 kDa to 36kDa and the 85 kDa glycosylated protein can be detected in SDS-PAGE. (PMID:11546643) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9525 Product name: Recombinant human SLC3A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 336-685 aa of BC022386 Sequence: IPDTVTQYSELYHDFTTTQVGMHDIVRSFRQTMDQYSTEPGRYRFMGTEAYAESIDRTVMYYGLPFIQEADFPFNNYLSMLDTVSGNSVYEVITSWMENMPEGKWPNWMIGGPDSSRLTSRLGNQYVNVMNMLLFTLPGTPITYYGEEIGMGNIVAANLNESYDINTLRSKSPMQWDNSSNAGFSEASNTWLPTNSDYHTVNVDVQKTQPRSALKLYQDLSLLHANELLLNRGWFCHLRNDSHYVVYTRELDGIDRIFIVVLNFGESTLLNLHNMISGLPAKIRIRLSTNSADKGSKVDTSGIFLDKGEGLIFEHNTKNLLHRQTAFRDRCFVSNRACYSSVLNILYTSC Predict reactive species Full Name: solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1 Calculated Molecular Weight: 685 aa, 79 kDa Observed Molecular Weight: 78-90 kDa GenBank Accession Number: BC022386 Gene Symbol: SLC3A1 Gene ID (NCBI): 6519 RRID: AB_2239419 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q07837 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.