Proteintech, 16395-1-AP, MOCOS Polyclonal antibody

Size: 20ul / 150ul The MOCOS (16395-1-AP) by Proteintech is a Polyclonal antibody targeting MOCOS in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 16395-1-AP targets MOCOS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells Positive IP detected in: HepG2 cells Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information MOCOS(Molybdenum cofactor sulfurase) is also named as MCS, MOS, hMCS and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. It catalyzes the removal of elemental sulfur from cysteine to produce alanine. Sulfation of molybdenum is essential for xanthine dehydrogenase (XDH) and aldehyde oxidase (ADO) enzymes in which molybdenum cofactor is liganded by 1 oxygen and 1 sulfur atom in active form. Defects in MOCOS are the cause of xanthinuria type 2 (XU2)(PMID:17368066). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9487 Product name: Recombinant human MOCOS protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 542-888 aa of BC012079 Sequence: WNNSSTVNAVPVAPPVCDVARTQPTPSEKAAGVLEGALGPHVVTNLYLYPIKSCAAFEVTRWPVGNQGLLYDRSWMVVNHNGVCLSQKQEPRLCLIQPFIDLRQRIMVIKAKGMEPIEVPLEENSERTQIRQSRVCADRVSTYDCGEKISSWLSTFFGRPCHLIKQSSNSQRNAKKKHGKDQLPGTMATLSLVNEAQYLLINTSSILELHRQLNTSDENGKEELFSLKDLSLRFRANIIINGKRAFEEEKWDEISIGSLRFQVLGPCHRCQMICIDQQTGQRNQHVFQKLSESRETKVNFGMYLMHASLDLSSPCFLSVGSQVLPVLKENVEGHDLPASEKHQDVTS Predict reactive species Full Name: molybdenum cofactor sulfurase Calculated Molecular Weight: 888 aa, 98 kDa Observed Molecular Weight: 98 kDa GenBank Accession Number: BC012079 Gene Symbol: MOCOS Gene ID (NCBI): 55034 RRID: AB_2297842 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96EN8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.