Proteintech, 16501-1-AP, DNAJB14 Polyclonal antibody

Size: 20ul / 150ul The DNAJB14 (16501-1-AP) by Proteintech is a Polyclonal antibody targeting DNAJB14 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 16501-1-AP targets DNAJB14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, HeLa cells, human testis tissue Positive IP detected in: Jurkat cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9668 Product name: Recombinant human DNAJB14 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-254 aa of BC022248 Sequence: MEGNRDEAEKCVEIAREALNAGNREKAQRFLQKAEKLYPLPSARALLEIIMKNGSTAGNSPHCRKPSGSGDQSKPNCTKDSTSGSGEGGKGYTKDQVDGVLSINKCKNYYEVLGVTKDAGDEDLKKAYRKLALKFHPDKNHAPGATDAFKKIGNAYAVLSNPEKRKQYDLTGNEEQACNHQNNGRFNFHRGCEADITPEDLFNIFFGGGFPSGSVHSFSNGRAGYSQQHQHRHSGHEREEERGDGGFSVFIQLM Predict reactive species Full Name: DnaJ (Hsp40) homolog, subfamily B, member 14 Calculated Molecular Weight: 379 aa, 43 kDa Observed Molecular Weight: 43 kDa GenBank Accession Number: BC022248 Gene Symbol: DNAJB14 Gene ID (NCBI): 79982 RRID: AB_2094414 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8TBM8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.