Proteintech, 16635-1-AP, SEPHS1 Polyclonal antibody

Size: 20ul / 150ul The SEPHS1 (16635-1-AP) by Proteintech is a Polyclonal antibody targeting SEPHS1 in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 16635-1-AP targets SEPHS1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, HepG2 cells, mouse liver tissue, T-47D cells Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human placenta tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information Selenophosphate synthetase 1 (SEPHS1) has been implicated in the maintenance of redox homeostasis in chondrocytes and its deficiency plays a causal role in the progression of osteoarthritis (PMID: 36202216). SEPHS1 downregulation in human umbilical vein cells (HUVECs) led to cell death rather than the inhibition of cell proliferation (PMID: 33622411). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9978 Product name: Recombinant human SEPHS1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-392 aa of BC000941 Sequence: MSTRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVMPRLGIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMMGRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDRERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIMPDNAVPGDVLVLTKPLGTQVAVAVHQWLDIPEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTAAGLMHTFNAHAATDITGFGILGHAQNLAKQQRNEVSFVIHNLPVLAKMAAVSKACGNMFGLMHGTCPETSGGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNPTPGATS Predict reactive species Full Name: selenophosphate synthetase 1 Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 43 kDa GenBank Accession Number: BC000941 Gene Symbol: SEPHS1 Gene ID (NCBI): 22929 RRID: AB_10603472 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P49903 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.