Proteintech, 16664-1-AP, AMZ2 Polyclonal antibody

Size: 20ul / 150ul The AMZ2 (16664-1-AP) by Proteintech is a Polyclonal antibody targeting AMZ2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 16664-1-AP targets AMZ2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, human brain tissue Positive IHC detected in: human liver tissue, human heart tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human spleen tissue, human ovary tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information AMZ2(archaelysin family metallopeptidase 2) is a zinc metalloprotease. Its calculated molecular weight and observed molecular weight are both 41kDa. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10141 Product name: Recombinant human AMZ2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-360 aa of BC056271 Sequence: MQIIRHSEQTLKTALISKNPVLVSQYEKLDAGEQRLMNEAFQPASDLFGPITLHSPSDWITSHPEAPQDFEQFFSDPYRKTPSPNKRSIYIQSIGSLGNTRIISEEYIKWLTGYCKAYFYGLRVKLLEPVPVSVTRCSFRVNENTHNLQIHAGDILKFLKKKKPEDAFCVVGITMIDLYPRDSWNFVFGQASLTDGVGIFSFARYGSDFYSMHYKGKVKKLKKTSSSDYSIFDNYYIPEITSVLLLRSCKTLTHEIGHIFGLRHCQWLACLMQGSNHLEEADRRPLNLCPICLHKLQCAVGFSIVERYKALVRWIDDESSDTPGATPEHSHEDNGNLPKPVEAFKEWKEWIIKCLAVLQK Predict reactive species Full Name: archaelysin family metallopeptidase 2 Calculated Molecular Weight: 360 aa, 41 kDa Observed Molecular Weight: 41 kDa GenBank Accession Number: BC056271 Gene Symbol: AMZ2 Gene ID (NCBI): 51321 RRID: AB_1850886 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86W34 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.