Proteintech, 16680-1-AP, RPS13 Polyclonal antibody

Size: 20ul / 150ul The RPS13 (16680-1-AP) by Proteintech is a Polyclonal antibody targeting RPS13 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 16680-1-AP targets RPS13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MCF-7 cells, mouse oterus tissue, rat oterus tissue Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information RPS13, also named as 40S ribosomal protein S13, belongs to the ribosomal protein S15P family. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. RPS13 is located in the cytoplasm. It is co-transcribed with two U14 small nucleolar RNA genes, which are located in its third and fifth introns. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10008 Product name: Recombinant human RPS13 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-151 aa of BC006772 Sequence: MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALVA Predict reactive species Full Name: ribosomal protein S13 Calculated Molecular Weight: 17 kDa Observed Molecular Weight: 14 kDa, 17 kDa GenBank Accession Number: BC006772 Gene Symbol: RPS13 Gene ID (NCBI): 6207 RRID: AB_2182500 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P62277 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.