Proteintech, 16836-1-AP, ATP1A1/2/3/4 Polyclonal antibody

Size: 20ul / 150ul The ATP1A1/2/3/4 (16836-1-AP) by Proteintech is a Polyclonal antibody targeting ATP1A1/2/3/4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 16836-1-AP targets ATP1A1/2/3/4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: 37°C incubated mouse heart tissue, 37°C incubated mouse skeletal muscle tissue Positive IHC detected in: mouse heart tissue, human kidney tissue, human testis tissue, human skin tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: C2C12 cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information ATP1A2 (Na+/K+-ATPase α-2 subunit) is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody was raised against the internal region of the human ATP1A2 and can recognizes all the isoforms of α subunit. The 65kDa band detected occasionally may be the degradation product of ATP1A2. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, canine, haliotis discus hannai Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10515 Product name: Recombinant human ATP1A2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 347-700 aa of BC052271 Sequence: KRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGATFDKRSPTWTALSRIAGLCNRAVFKAGQENISVSKRDTAGDASESALLKCIELSCGSVRKMRDRNPKVAEIPFNSTNKYQLSIHEREDSPQSHVLVMKGAPERILDRCSTILVQGKEIPLDKEMQDAFQNAYMELGGLGERVLGFCQLNLPSGKFPRGFKFDTDELNFPTEKLCFVGLMSMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPMSQVNPREAKACVVHGSDLKDMTSEQLDEILKNHTEIVFARTSPQQKLIIVE Predict reactive species Full Name: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide Calculated Molecular Weight: 1020 aa, 112 kDa Observed Molecular Weight: 97-100 kDa GenBank Accession Number: BC052271 Gene Symbol: ATP1A2 Gene ID (NCBI): 477 RRID: AB_2061149 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P50993 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.