Proteintech, 17032-1-AP, GRK5 Polyclonal antibody

Size: 20ul / 150ul The GRK5 (17032-1-AP) by Proteintech is a Polyclonal antibody targeting GRK5 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 17032-1-AP targets GRK5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, human skeletal muscle tissue, HepG2 cells，mouse skeletal muscle tissue, rat skeletal muscle tissue Positive IHC detected in: human skeletal muscle tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information GRK5, also named as GPRK5, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. It is Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. GRK5 is crucial for proper neuronal morphogenesis and the establishment of functional neuronal circuitry. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10511 Product name: Recombinant human GRK5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 186-590 aa of BC064506 Sequence: FRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSICKMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVSIPWQNEMIETECFKELNVFGPNGTLPPDLNRNHPPEPPKKGLLQRLFKRQHQNNSKSSPSSKTSFNHHINSNHVSSNSTGSS Predict reactive species Full Name: G protein-coupled receptor kinase 5 Calculated Molecular Weight: 590 aa, 68 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC064506 Gene Symbol: GRK5 Gene ID (NCBI): 2869 RRID: AB_2115453 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P34947 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.