Proteintech, 17040-1-AP, METAP2 Polyclonal antibody

Size: 20ul / 150ul The METAP2 (17040-1-AP) by Proteintech is a Polyclonal antibody targeting METAP2 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 17040-1-AP targets METAP2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, MCF-7 cells, mouse pancreas tissue, mouse testis tissue Positive IP detected in: MCF-7 cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information MetAP2, also known as MAP2, P67EIF2 or p67, is one of the three known methionine aminopeptidases responsible for the removal of the N-terminal translation initiator methionine from newly synthesized proteins, which is a critical step in protein maturation (PMID: 27692546, 16568094). The enzyme binds two cobalt or manganese ions in its active site (PMID: 11524009, 12718546). As higher MetAP2 expression was observed in tumor (mesothelioma, neuroblastoma, and colorectal carcinoma) cells compared with normal cells, the enzyme serves as a target for anti-angiogenic compounds of natural origin, such as fumagillin and ovalicin (PMID: 11485930, 15927358, 15102683, 9224570). Selective inhibition of MetAP2 stops vascularization and tumor growth in animal models (PMID: 12118091). MetAP2 has 3 isoforms with molecular mass of 50-53 kDa and apparent molecular mass 67 kD. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10564 Product name: Recombinant human METAP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 132-478 aa of BC013782 Sequence: GQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY Predict reactive species Full Name: methionyl aminopeptidase 2 Calculated Molecular Weight: 478 aa, 53 kDa Observed Molecular Weight: 67 kDa GenBank Accession Number: BC013782 Gene Symbol: METAP2 Gene ID (NCBI): 10988 RRID: AB_2144162 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P50579 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.