Proteintech, 17084-1-AP, RAI1 Polyclonal antibody

Size: 20ul / 150ul The RAI1 (17084-1-AP) by Proteintech is a Polyclonal antibody targeting RAI1 in WB, IHC, ELISA applications with reactivity to human samples 17084-1-AP targets RAI1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The RAI1 (Retinoic Acid Induced 1) gene is located on the short arm of chromosome 17. The RAI1 protein it encodes is a crucial transcriptional regulator, playing a central role in processes such as neurodevelopment, sleep regulation, circadian rhythms, and energy metabolism. This protein contains a chromatin domain that binds to specific DNA sequences and recruits other transcriptional co-factors to activate or repress the expression of downstream target genes. The RAI1 gene is extremely sensitive to haploinsufficiency, and its dysfunction is closely linked to several severe neurodevelopmental disorders. For instance, RAI1 haploinsufficiency causes Smith-Magenis syndrome, while duplication of the RAI1 gene is associated with Potocki-Lupski syndrome. Consequently, the RAI1 protein is a key molecule for understanding brain function and behavioral regulation, and a major focus of research for related genetic syndromes. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9046 Product name: Recombinant human RAI1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC021209 Sequence: MQSFRERCGFHGKQQNYQQTSQETSRLENYRQPSQAGLSCDRQRLLAKDYYNPQPYPSYEGGAGTPSGTAAAVAADKYHRGSKALPTQQGLQGRPAFPGYGVQDSSPYPGRYAGEESLQAWGAPQPPPPQPQPLPAGVAKYDENLMKKTAVPPSRQYAEQGAQVPFRTHSLHVQQPPPPQQPLAYPKLQRQKLQNDIASPLPFPQGTHFPQHSQSFPTSSTYSSSVQGGGQGAHSYKSCTAPTAQPHDRPLTASSSLAPGQRVQNLHAYQSGRLSYDQQQQQQQQQQQQQQALQSRHHAQETLHYQNLAKYQHYGQQGQGYCQPDAAVRTPEQYYQTFSPSSSHSPARSV Predict reactive species Full Name: retinoic acid induced 1 Calculated Molecular Weight: 1906aa,203 kDa; 966aa,104 kDa Observed Molecular Weight: 250-260 kDa GenBank Accession Number: BC021209 Gene Symbol: RAI1 Gene ID (NCBI): 10743 RRID: AB_2284824 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q7Z5J4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.