Proteintech, 17146-1-AP, PYCR2 Polyclonal antibody

Size: 20ul / 150ul The PYCR2 (17146-1-AP) by Proteintech is a Polyclonal antibody targeting PYCR2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 17146-1-AP targets PYCR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, L02 cells Positive IHC detected in: human breast cancer tissue, human placenta tissue, human kidney tissue, human testis tissue, human lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1500-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, african green monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10918 Product name: Recombinant human PYCR2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-320 aa of BC020553 Sequence: MSVGFIGAGQLAYALARGFTAAGILSAHKIIASSPEMNLPTVSALRKMGVNLTRSNKETVKHSDVLFLAVKPHIIPFILDEIGADVQARHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVVVQEGATVYATGTHALVEDGQLLEQLMSSVGFCTEVEEDLIDAVTGLSGSGPAYAFMALDALADGGVKMGLPRRLAIQLGAQALLGAAKMLLDSEQHPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKISPAALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD Predict reactive species Full Name: pyrroline-5-carboxylate reductase family, member 2 Calculated Molecular Weight: 320 aa, 34 kDa Observed Molecular Weight: 34 kDa GenBank Accession Number: BC020553 Gene Symbol: PYCR2 Gene ID (NCBI): 29920 RRID: AB_2253344 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96C36 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.