Proteintech, 17150-1-AP, YARS2 Polyclonal antibody

Size: 20ul / 150ul The YARS2 (17150-1-AP) by Proteintech is a Polyclonal antibody targeting YARS2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 17150-1-AP targets YARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: K-562 cells, HepG2 cells Positive IP detected in: K-562 cells Positive IHC detected in: human testis tissue, human placenta tissue, human skin tissue, human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9530 Product name: Recombinant human YARS2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 128-477 aa of BC015625 Sequence: EREALETERVRANARALRLGLEALAANHQQLFTDGRSWGSFTVLDNSAWYQKQHLVDFLAAVGGHFRMGTLLSRQSVQLRLKSPEGMSLAEFFYQVLQAYDFYYLFQRYGCRVQLGGSDQLGNIMSGYEFINKLTGEDVFGITVPLITSTTGAKLGKSAGNAVWLNRDKTSPFELYQFFVRQPDDSVERYLKLFTFLPLPEIDHIMQLHVKEPERRGPQKRLAAEVTKLVHGREGLDSAKRCTQALYHSSIDALEVMSDQELKELFKEAPFSEFFLDPGTSVLDTCRKANAIPDGPRGYRMITEGGVSINHQQVTNPESVLIVGQHILKNGLSLLKIGKRNFYIIKWLQL Predict reactive species Full Name: tyrosyl-tRNA synthetase 2, mitochondrial Calculated Molecular Weight: 477 aa, 53 kDa Observed Molecular Weight: 45-50 kDa GenBank Accession Number: BC015625 Gene Symbol: YARS2 Gene ID (NCBI): 51067 RRID: AB_2219263 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y2Z4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.