Proteintech, 17206-1-AP, HMGCLL1 Polyclonal antibody

Size: 20ul / 150ul The HMGCLL1 (17206-1-AP) by Proteintech is a Polyclonal antibody targeting HMGCLL1 in WB, IHC, ELISA applications with reactivity to human samples 17206-1-AP targets HMGCLL1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: SKOV-3 cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information HMGCLL1 (3-hydroxymethyl-3-methylglutaryl-CoA lyase-like protein 1), also named as er-cHL, is a close homolog of mitochondrial HMG-CoA lyase (mHL). HMGCLL1 catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, which is a key step in ketogenesis (PMID: 22847177, 22865860). HMGCLL1 has some isoforms with the molecular mass of 32-39 kDa and 6 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10985 Product name: Recombinant human HMGCLL1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-340 aa of BC024194 Sequence: MGNVPSAVKHCLSYQQLLREHLWIGDSVAGALDPAQETSQLSGLPEFVKIVEVGPRDGLQNEKVIVPTDIKIEFINRLSQTGLSVIEVTSFVSSRWVPQMADHTEVMKGIHQYPGVRYPVLTPNLQGFHHAVAAGATEISVFGAASESFSKKNINCSIEESMGKFEEVVKSARHMNIPARGYVSCALGCPYEGSITPQKVTEVSKRLYGMGCYEISLGDTIGVGTPGSMKRMLESVMKEIPPGALAVHCHDTYGQALANILTALQMGINVVDSAVSGLGGCPYAKGASGNVATEDLIYMLNGLGLNTGVNLYKVMEAGDFICKAVNKTTNSKVAQASFNA Predict reactive species Full Name: 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase-like 1 Calculated Molecular Weight: 340 aa, 36 kDa Observed Molecular Weight: 32 kDa GenBank Accession Number: BC024194 Gene Symbol: HMGCLL1 Gene ID (NCBI): 54511 RRID: AB_2878362 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8TB92 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.