Proteintech, 17222-1-AP, RIOK1 Polyclonal antibody

Size: 20ul / 150ul The RIOK1 (17222-1-AP) by Proteintech is a Polyclonal antibody targeting RIOK1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 17222-1-AP targets RIOK1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells Positive IP detected in: HeLa cells Positive IHC detected in: rat stomach tissue, human placenta tissue, human stomach cancer tissue, mouse cerebellum tissue, rat cerebellum tissue, rat skin tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information RIOK1 (RIO Kinase 1) is a protein-coding gene located on chromosome 6 in humans. It encodes a serine/threonine-protein kinase that belongs to the RIO family of atypical protein kinases/ATPases. This family also includes RIOK2, RIOK3, and RIOB, which have evolved from Archaea to humans. RIOK1 is best known for its role in ribosome biogenesis and its involvement in various cellular processes, including translation regulation and cell-cycle progression. (PMID: 37301535, PMID: 37935656) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11057 Product name: Recombinant human RIOK1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 219-568 aa of BC006104 Sequence: DRDKYVSGEFRFRHGYCKGNPRKMVKTWAEKEMRNLIRLNTAEIPCPEPIMLRSHVLVMSFIGKDDMPAPLLKNVQLSESKARELYLQVIQYMRRMYQDARLVHADLSEFNMLYHGGGVYIIDVSQSVEHDHPHALEFLRKDCANVNDFFMRHSVAVMTVRELFEFVTDPSITHENMDAYLSKAMEIASQRTKEERSSQDHVDEEVFKRAYIPRTLNEVKNYERDMDIIMKLKEEDMAMNAQQDNILYQTVTGLKKDLSGVQKVPALLENQVEERTCSDSEDIGSSECSDTDSEEQGDHARPKKHTTDPDIDKKERKKMVKEAQREKRKNKIPKHVKKRKEKTAKTKKGK Predict reactive species Full Name: RIO kinase 1 (yeast) Calculated Molecular Weight: 568 aa, 66 kDa Observed Molecular Weight: 75-80 kDa GenBank Accession Number: BC006104 Gene Symbol: RIOK1 Gene ID (NCBI): 83732 RRID: AB_2284990 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BRS2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.