Proteintech, 17254-1-AP, BBX Polyclonal antibody

Size: 20ul / 150ul The BBX (17254-1-AP) by Proteintech is a Polyclonal antibody targeting BBX in WB, IP, IHC, ELISA applications with reactivity to human samples 17254-1-AP targets BBX in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells Positive IP detected in: HepG2 cells Positive IHC detected in: human ovary tissue, human brain tissue, human lung tissue, human placenta tissue, human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information BBX, also named HBP2, is a protein with a high mobility group (HMG) domain that belongs to the Sox (Sry-related HMG box) family of transcription factors. As a DNA-binding domain in HBP2, HMG is essential for its function in Schizosaccharomyces pombe. HBP2 activates CdcIO-dependent transcription at G1/s and allows growth of cdc10-129, resldelta and rep2delta mutant cells at the restrictive temperature Specification Tested Reactivity: human Cited Reactivity: mouse, rat, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10691 Product name: Recombinant human BBX protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 249-597 aa of BC012837 Sequence: KPEDSDCHRKIETCGSRKSERSCKGALYKTLVSEGMLTSLRANVDRGKRSSGKGNSSDHEGCWNEESWTFSQSGTSGSKKFKKTKPKEDCLLGSAKLDEEFEKKFNSLPQYSPVTFDRKCVPVPRKKKKTGNVSSEPTKTSKGPFQSQKKNLFHKIVSKYKHKKEKPNVPEKGSGDKWSNKQLFLDAIHPTEAIFSEDRNTMEPVHKVKNIPSIFNTPEPTTTQEPLVGSQKRKARKTKITHLVRTADGRVSPAGGTLDDKPKEQLQRSLPKATETDCNDKCSHNTEVGETRSSTPEMPAVSAFFSLAALAEVAAMENVHRGQRSTPLTHDGQPKEMPQAPVLISCADQ Predict reactive species Full Name: bobby sox homolog (Drosophila) Calculated Molecular Weight: 941 aa, 105 kDa Observed Molecular Weight: 105 kDa GenBank Accession Number: BC012837 Gene Symbol: BBX Gene ID (NCBI): 56987 RRID: AB_2065285 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8WY36 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.