Proteintech, 17270-1-AP, POLR2I Polyclonal antibody

Size: 20ul / 150ul The POLR2I (17270-1-AP) by Proteintech is a Polyclonal antibody targeting POLR2I in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 17270-1-AP targets POLR2I in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells Positive IHC detected in: mouse stomach tissue, rat liver tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information POLR2I, also known as RPB9 or hRPB14.5, is a small core subunit of RNA polymerase II involved in DNA repair and many transcription-related processes (PMID: 21450810). It is expressed in many tissues and may play a key role in the pathological process of hypertensive nephropathy (PMID: 34422001). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11027 Product name: Recombinant human POLR2I protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-125 aa of BC017112 Sequence: MEPDGTYEPGFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE Predict reactive species Full Name: polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa Calculated Molecular Weight: 125 aa, 15 kDa Observed Molecular Weight: 15 kDa GenBank Accession Number: BC017112 Gene Symbol: POLR2I Gene ID (NCBI): 5438 RRID: AB_10836936 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P36954 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.