Proteintech, 17333-1-AP, HSL Polyclonal antibody

Size: 20ul / 150ul The HSL (17333-1-AP) by Proteintech is a Polyclonal antibody targeting HSL in WB, IHC, ELISA applications with reactivity to human, mouse, pig samples 17333-1-AP targets HSL in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, pig samples. Tested Applications Positive WB detected in: mouse adipose tissue, pig adipose tissue Positive IHC detected in: mouse brown adipose tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Hormone sensitive lipase (HSL) belongs to the 'GDXG' lipolytic enzyme family. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. HSL has two isoforms with the molecular mass of 117 kDa and 84 kDa. Specification Tested Reactivity: human, mouse, pig Cited Reactivity: human, mouse, rat, pig, monkey, zebrafish, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11360 Product name: Recombinant human LIPE protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 728-1076 aa of BC070041 Sequence: GNLCFTVALRAAAYGVRVPDGIMAAYPATMLQPAASPSRLLSLMDPLLPLSVLSKCVSAYAGAKTEDHSNSDQKALGMMGLVRRDTALLLRDFRLGASSWLNSFLELSGRKSQKMSEPIAEPMRRSVSEAALAQPQGPLGTDSLKNLTLRDLSLRGNSETSSDTPEMSLSAETLSPSTPSDVNFLLPPEDAGEEAEAKNELSPMDRGLGVRAAFPEGFHPRRSSQGATQMPLYSSPIVKNPFMSPLLAPDSMLKSLPPVHIVACALDPMLDDSVMLARRLRNLGQPVTLRVVEDLPHGFLTLAALCRETRQAAELCVERIRLVLTPPAGAGPSGETGAAGVDGGCGGRH Predict reactive species Full Name: lipase, hormone-sensitive Calculated Molecular Weight: 117 kDa Observed Molecular Weight: 84 kDa GenBank Accession Number: BC070041 Gene Symbol: HSL Gene ID (NCBI): 3991 RRID: AB_2878386 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q05469 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.