Proteintech, 17352-1-AP, EFHB Polyclonal antibody

Size: 20ul / 150ul The EFHB (17352-1-AP) by Proteintech is a Polyclonal antibody targeting EFHB in IHC, IF-P, ELISA applications with reactivity to human, mouse samples 17352-1-AP targets EFHB in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse testis tissue Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information EFHB (EF-hand domain family member B, also known as CFAP21) interacts with Orai1 in a Ca2+-dependent manner. Human EFHB is an 833-amino acid cytosolic protein with two EF-hand domains between amino acids 561-596 and 597-632 (PMID: 34439314). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11431 Product name: Recombinant human EFHB protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 319-669 aa of BC028198 Sequence: RADDFKEKFQHKLGRVLDPIAETMNVPPDCTFGACLRPEEYGVGDLIHNRLPDEYLRGKDRQRALIAAVRHHLKKVNYQKFDTLLAAFRHYDKKGDGMIDKDELQEACDQANLSLDDKLLDQLFDYCDVDNDGFINYLEFANFLNWKDKMLLKEYEERVIIKGRKPDCVNPTEANVEEPEQTLLIKPEDIVLKEAGSTEKTLRTLLRPSDKVSNYYKTTSSEINAIVGAIPSTCYPICGVPTIRSDIPAPRIRRISDRTNYGEEGSAYSLLYPTIFARKGVFERDFFKTRSKEEIAEILCNIGVKLSDEEFENVWNLASKKHHRGEVCVENIRNVLDELRHADRIKCKTLM Predict reactive species Full Name: EF-hand domain family, member B Calculated Molecular Weight: 669aa,76 kDa; 831aa,94 kDa GenBank Accession Number: BC028198 Gene Symbol: EFHB Gene ID (NCBI): 151651 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N7U6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.