Proteintech, 17464-1-AP, FASTKD2 Polyclonal antibody

Size: 20ul / 150ul The FASTKD2 (17464-1-AP) by Proteintech is a Polyclonal antibody targeting FASTKD2 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 17464-1-AP targets FASTKD2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells Positive IP detected in: HepG2 cells Positive IF/ICC detected in: COS7 cells, U2OS cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information FASTKD2 encodes a FAS-activated serine-threonine (FAST) kinase domain 2 which locates in the mitochondrial inner compartment. FASTKD2 is a member of the human FASTK protein family, formed by six architecturally related proteins named FASTK and FASTKD1-5 that localize to mitochondria. The predicted MW of FASTKD2 is 81 kDa, yet the western blot of FASTKD2 revealed a band of ~60 kDa. (PMID: 26370583, PMID: 26990993, PMID: 25683715) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11314 Product name: Recombinant human FASTKD2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 361-710 aa of BC001544 Sequence: ILLDECSKVVLDNIHGCPLRIMINILQSCKDLQYHNLDLFKGLADYVAATFDIWKFRKVLFILILFENLGFRPVGLMDLFMKRIVEDPESLNMKNILSILHTYSSLNHVYKCQNKEQFVEVMASALTGYLHTISSENLLDAVYSFCLMNYFPLAPFNQLLQKDIISELLTSDDMKNAYKLHTLDTCLKLDDTVYLRDIALSLPQLPRELPSSHTNAKVAEVLSSLLGGEGHFSKDVHLPHNYHIDFEIRMDTNRNQVLPLSDVDTTSATDIQRVAVLCVSRSAYCLGSSHPRGFLAMKMRHLNAMGFHVILVNNWEMDKLEMEDAVTFLKTKIYSVEALPVAAVNVQSTQ Predict reactive species Full Name: FAST kinase domains 2 Calculated Molecular Weight: 710 aa, 81 kDa Observed Molecular Weight: 55-65 kDa GenBank Accession Number: BC001544 Gene Symbol: FASTKD2 Gene ID (NCBI): 22868 RRID: AB_2101119 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NYY8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.