Proteintech, 17509-1-AP, IRS1 Polyclonal antibody

Size: 20ul / 150ul The IRS1 (17509-1-AP) by Proteintech is a Polyclonal antibody targeting IRS1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human samples 17509-1-AP targets IRS1 in WB, IHC, IF/ICC, FC (Intra), IP, Dot blot, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, A431 cells, HEK-293 cells, PC-3 cells Positive IP detected in: A549 cells Positive IHC detected in: human colon tissue, human breast cancer tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Positive FC (Intra) detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Ins receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in ins receptor (IR) and ins-like growth factor I receptor (IGF-IR) signaling (PMID: 10669726). IRS1 is phosphorylated by ins receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression (PMID: 11259577). Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance (). IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, pig, megalobrama amblycephala Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11714 Product name: Recombinant human IRS1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 895-1243 aa of BC053895 Sequence: EYVNIEFGSDQSGYLSGPVAFHSSPSVRCPSQLQPAPREEETGTEEYMKMDLGPGRRAAWQESTGVEMGRLGPAPPGAASICRPTRAVPSSRGDYMTMQMSCPRQSYVDTSPAAPVSYADMRTGIAAEEVSLPRATMAAASSSSAASASPTGPQGAAELAAHSSLLGGPQGPGGMSAFTRVNLSPNRNQSAKVIRADPQGCRRRHSSETFSSTPSATRVGNTVPFGAGAAVGGGGGSSSSSEDVKRHSSASFENVWLRPGELGGAPKEPAKLCGAAGGLENGLNYIDLDLVKDFKQCPQECTPEPQPPPPPPPHQPLGSGESSSTRRSSEDLSAYASISFQKQPEDRQ Predict reactive species Full Name: insulin receptor substrate 1 Calculated Molecular Weight: 1242 aa, 132 kDa Observed Molecular Weight: 160-185 kDa GenBank Accession Number: BC053895 Gene Symbol: IRS1 Gene ID (NCBI): 3667 RRID: AB_10596914 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P35568 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.