Proteintech, 17533-1-AP, DNAI2 Polyclonal antibody

Size: 20ul / 150ul The DNAI2 (17533-1-AP) by Proteintech is a Polyclonal antibody targeting DNAI2 in WB, IHC, IF-P, IP, ELISA applications with reactivity to human, mouse, rat samples 17533-1-AP targets DNAI2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse ovary tissue, HeLa cells, mouse lung tissue, mouse testis tissue Positive IP detected in: mouse lung tissue Positive IHC detected in: mouse ovary tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse testis tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11585 Product name: Recombinant human DNAI2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 239-593 aa of BC039582 Sequence: GQIACWDTRKGSLVAELSTIESSHRDPVYGTIWLQSKTGTECFSASTDGQVMWWDIRKMSEPTEVVILDITKKEQLENALGAISLEFESTLPTKFMVGTEQGIVISCNRKAKTSAEKIVCTFPGHHGPIYALQRNPFYPKNFLTVGDWTARIWSEDSRESSIMWTKYHMAYLTDAAWSPVRPTVFFTTRMDGTLDIWDFMFEQCDPTLSLKDNGCLIACGSQLGTTTLLEVSPGLSTLQRNEKNVASSMFERETRREKILEARHREMRLKEKGKAEGRDEEQTDEELAVDLEALVSKAEEEFFDIIFTELKKKEADAIKLTPVPQQPSPEEDQVVEEGEEAAGEEGDEEVEEDLA Predict reactive species Full Name: dynein, axonemal, intermediate chain 2 Calculated Molecular Weight: 593 aa, 67 kDa Observed Molecular Weight: 70-80 kDa GenBank Accession Number: BC039582 Gene Symbol: DNAI2 Gene ID (NCBI): 64446 RRID: AB_2096670 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9GZS0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.