Proteintech, 17541-1-AP, ARAP1 Polyclonal antibody

Size: 20ul / 150ul The ARAP1 (17541-1-AP) by Proteintech is a Polyclonal antibody targeting ARAP1 in WB, IHC, ELISA applications with reactivity to human samples 17541-1-AP targets ARAP1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, K-562 cells, L02 cells Positive IHC detected in: human kidney tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ARAP1 is a member of ARAP family with both Arf GAP and Rho GAP activities, by which ARAP1 makes a direct link between ADP-ribosylation factor (Arf) GTPases and Rho GTPases signaling. ARAP1 is involved in membrane trafficking and receptor recycling. It supports the plasma membrane recycling of the angiotensin II type 1 receptor (AT1) and regulates the cell-specific trafficking of the pro-death receptor TRAIL-R1/DR4, thereby promoting TRAIL-induced apoptosis in certain cell types. (PMID: 14559250, PMID: 18165900, PMID: 38008882) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11597 Product name: Recombinant human ARAP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 329-679 aa of BC056401 Sequence: LYCVQCFSDTNQMNVHNLAIVFGPTLFQTDGQDYKAGRVVEDLINHYVVVFSVDEEELRKQREEITAIVKMRVAGTASGTQHAGDFICTVYLEEKKAETEQHIKVPASMTAEELTLEILDRRNVGIREKDYWTCFEVNEREEAERPLHFAEKVLPILHGLGTDSHLVVKKHQAMEAMLLYLASRVGDTKHGMMKFREDRSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSHRPEKEWPIKSLKVYLGVKKKLRPPTCWGFTVVHETEKHEKQQWYLCCDTQMELREWFATFLFVQHDGLVWPSEPSRMSRAVPEVRLGSVSLIPLRGSENEMRRSVAAFTADPLSLLRNV Predict reactive species Full Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 Calculated Molecular Weight: 162 kDa Observed Molecular Weight: 180 kDa GenBank Accession Number: BC056401 Gene Symbol: ARAP1 Gene ID (NCBI): 116985 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96P48 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.