Proteintech, 17575-1-AP, MDM1 Polyclonal antibody

Size: 20ul / 150ul The MDM1 (17575-1-AP) by Proteintech is a Polyclonal antibody targeting MDM1 in WB, IHC, IF/ICC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 17575-1-AP targets MDM1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse retina tissue, HEK-293 cells, Raji cells, rat retina tissue Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse eye tissue Positive IF/ICC detected in: hTERT-RPE1 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Mdm1 was upregulated during ciliogenesis in multiciliated tracheal epithelial cells and localized to centrioles and cilia when transfected into NIH/3T3 cells. Mdm1 is a microtubule-binding protein that inhibits centrosome duplication when overexpressed. Proteomic analysis revealed that Mdm1 was also detected in the mouse photoreceptor sensory cilium complex and the outer segment (OS) of photoreceptor cells in the retina, suggesting that Mdm1 might be involved in the regulation of the functions and structure of the centrosome, as well as cilia. (PMID: 36171205) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10514 Product name: Recombinant human MDM1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 365-714 aa of BC028355 Sequence: SDSNCCWDVSSTTSSEGTVSSNIRALDLAGDPTSHKTLQKCPSTEPEEKGNIVEEQPQKNTTEKLGVSAPTIPVRRRLAWDTENTSEDVQKQPGEKEEEDDNEEEGDRKTGKQAFMGEQEKLDVHEKSKADKMKEGSDSSVSSEKGGRLPTPKLRELGGIQRTHHDLTTPAVGGAVLVSPSKMKPPALEQRKRMTSQDCLETSKNDFTKKESRAVSLLTSPAAGIKTVDPLPLREDSEDNIHKFAEATLPVSKIPKYPTNPPGQLPSPPHVPSYWHPSRRIQGSLRDPEFQHNVGKARMNNLQLPQHEAFNDEDEDRLSEISARSAASSLRAFQTLARAKKRKENFWGKT Predict reactive species Full Name: Mdm1 nuclear protein homolog (mouse) Calculated Molecular Weight: 81 kDa Observed Molecular Weight: 81 kDa GenBank Accession Number: BC028355 Gene Symbol: MDM1 Gene ID (NCBI): 56890 RRID: AB_2281929 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8TC05 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.