Proteintech, 17673-1-AP, VPS4A/B Polyclonal antibody

Size: 20ul / 150ul The VPS4A/B (17673-1-AP) by Proteintech is a Polyclonal antibody targeting VPS4A/B in WB, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 17673-1-AP targets VPS4A/B in WB, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, mouse skin tissue, HEK-293 cells, HeLa cells, NIH/3T3 cells Positive IP detected in: HeLa cells Positive FC (Intra) detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. Human VPS4 has two orthologous proteins named VPS4A and VPS4B. This antibody can recognize both VPS4A and VPS4B due to their high homology. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11610 Product name: Recombinant human VPS4B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-444 aa of BC039574 Sequence: MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQEG Predict reactive species Full Name: vacuolar protein sorting 4 homolog B (S. cerevisiae) Calculated Molecular Weight: 444 aa, 49 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC039574 Gene Symbol: VPS4B Gene ID (NCBI): 9525 RRID: AB_2215504 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75351 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.