Proteintech, 17711-1-AP, ARL13B Polyclonal antibody

Size: 20ul / 150ul The ARL13B (17711-1-AP) by Proteintech is a Polyclonal antibody targeting ARL13B in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat, canine samples 17711-1-AP targets ARL13B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples. Tested Applications Positive WB detected in: L02 cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue, NIH/3T3 cells, rat liver tissue, rat kidney tissue Positive IP detected in: L02 cells Positive IHC detected in: mouse brain tissue, human liver tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: hTERT-RPE1 cells, NIH3T3 cells, MDCK cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information ARL13B, also named ARL2L1, is the Ras superfamily's small ciliary G protein. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B cause Joubert syndrome (JS), an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band will likely represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986). Specification Tested Reactivity: human, mouse, rat, canine Cited Reactivity: human, mouse, rat, pig, canine, monkey, chicken, zebrafish, sheep, xenopus Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12015 Product name: Recombinant human ARL13B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-321 aa of BC094725 Sequence: MFSLMASCCGWFKRWREPVRLANKQDKEGALGEADVIECLSLEKLVNEHKCLCQIEPCSAISGYGKKIDKSIKKGLYWLLHVIARDFDALNERIQKETTEQRALEEQEKQERAERVRKLREERKQNEQEQAELDGTSGLAELDPEPTNPFQPIASVIIENEGKLEREKKNQKMEKDSDGCHLKHKMEHEQIETQGQVNHNGQKNNEFGLVENYKEALTQQLKNEDETDRPSLESANGKKKTKKLRMKRNHRVEPLNIDDCAPESPTPPPPPPPVGWGTPKVTRLPKLEPLGETHHNDFYRKPLPPLAVPQRPNSDAHDVIS Predict reactive species Full Name: ADP-ribosylation factor-like 13B Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 40-48 kDa, 60 kDa GenBank Accession Number: BC094725 Gene Symbol: ARL13B Gene ID (NCBI): 200894 RRID: AB_2060867 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q3SXY8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.