Proteintech, 17720-1-AP, CSK Polyclonal antibody

Size: 20ul / 150ul The CSK (17720-1-AP) by Proteintech is a Polyclonal antibody targeting CSK in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, rat samples 17720-1-AP targets CSK in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: Raji cells, K-562 cells, HL-60 cells, Jurkat cells, rat lung tissue Positive IP detected in: HL-60 cells Positive IHC detected in: human lung tissue, human skin tissue, human placenta tissue, human kidney tissue, human brain tissue, human ovary tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701). Specification Tested Reactivity: human, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12103 Product name: Recombinant human CSK protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 83-450 aa of BC106073 Sequence: FHGKITREQAERLLYPPETGLFLVRESTNYPGDYTLCVSCDGKVEHYRIMYHASKLSIDEEVYFENLMQLVEHYTSDADGLCTRLIKPKVMEGTVAAQDEFYRSGWALNMKELKLLQTIGKGEFGDVMLGDYRGNKVAVKCIKNDATAQAFLAEASVMTQLRHSNLVQLLGVIVEEKGGLYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSEDNVAKVSDFGLTKEASSTQDTGKLPVKWTAPEALREKKFSTKSDVWSFGILLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYEVMKNCWHLDAAMRPSFLQLREQLEHIKTHELHL Predict reactive species Full Name: c-src tyrosine kinase Calculated Molecular Weight: 450 aa, 50 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC106073 Gene Symbol: CSK Gene ID (NCBI): 1445 RRID: AB_2084150 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P41240 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.