Proteintech, 17723-1-AP, SAMD4B Polyclonal antibody

Size: 20ul / 150ul The SAMD4B (17723-1-AP) by Proteintech is a Polyclonal antibody targeting SAMD4B in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 17723-1-AP targets SAMD4B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HT-29 cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue, HeLa cells Positive IHC detected in: human urothelial carcinoma tissue, human thyroid cancer tissue, mouse kidney tissue, mouse pancreas tissue, rat kidney tissue, rat pancreas tissue, rat small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information Sterile alpha motif domain-containing protein 4B (SAMD4B) is also named as Smaug2. SAMD4B is a mammalian homologue of the Smaug protein of Drosophila melanogaster and can participate in the process of regulating RNA stability as a posttranscriptional inhibitor (PMID: 23592795). SAMD4B is expressed in most human embryos and adult tissue (PMID: 23592795). SAMD4B, especially its expression level, could affect the immune microenvironment (PMID: 38886351). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12113 Product name: Recombinant human SAMD4B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 343-694 aa of BC054518 Sequence: KGARHKIALSIQKLRERQSVLKSLEKDVLEGGNLRNALQELQQIIITPIKAYSVLQATVAAATTTPTAKDGAPGEPPLPGAEPPLAHPGTDKGTEAKDPPAVENYPPPPAPAPTDGSEPAPAPVADGDIPSQFTRVMGKVCTQLLVSRPDEENITSYLQLIEKCLTHEAFTETQKKRLLSWKQQVLKLLRTFPRKAALEMQNYRQQKGWAFGSNSLPIAGSVGMGVARRTQRQFPMPPRALPPGRMGLLSPSGIGGVSPRHALTSPSLGGQGRQNLWFANPGGSNSMPSQSRSSVQRTHSLPVHSSPQAILMFPPDCPVPGPDLEINPTLESLCLSMTEHALGDGTDKTSTI Predict reactive species Full Name: sterile alpha motif domain containing 4B Calculated Molecular Weight: 694 aa, 75 kDa Observed Molecular Weight: 70-75 kDa GenBank Accession Number: BC054518 Gene Symbol: SAMD4B Gene ID (NCBI): 55095 RRID: AB_2183381 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q5PRF9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.