Proteintech, 17742-1-AP, DSN1 Polyclonal antibody

Size: 20ul / 150ul The DSN1 (17742-1-AP) by Proteintech is a Polyclonal antibody targeting DSN1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 17742-1-AP targets DSN1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information DSN1, also named as C20orf172 and MIS13, is required to shape an interface between the Mis12 complex and Blinkin, the counterpart of Spc105. DSN1 is part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. DSN1 has four isoforms with MW 27-33 kDa and 38-40 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11988 Product name: Recombinant human DSN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-340 aa of BC026011 Sequence: MSKTHDHQLESSLSPVEAFAKTSASLEMNQGVSEERIHLGSSPKKGGNCDLSHQERLQSKSLHLSPQEQSASYQDRRQSWRRASMKETNRRKSLHPIHQGITELSRSISVDLAESKRLGCLLLSSFQFSIQKLEPFLRDTKGFSLESFRAKASSLSEELKHFADGLETDGTLQKCFEDSNGKASDFSLEASVAEMKEYITKFSLERQTWDQLLLHYQQEAKEILSRGSTEAKITEVKVEPMTYLGSSQNEVLNTKPDYQKILQNQSKVFDCMELVMDELQGSVKQLQAFMDESTQCFQKVSVQLGKRSMQQLDPSPARKLLKLQLQNPPAIHGSGSGSCQ Predict reactive species Full Name: DSN1, MIND kinetochore complex component, homolog (S. cerevisiae) Calculated Molecular Weight: 340 aa, 38 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC026011 Gene Symbol: DSN1 Gene ID (NCBI): 79980 RRID: AB_2878431 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H410 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.