Proteintech, 17758-1-AP, DCK Polyclonal antibody

Size: 20ul / 150ul The DCK (17758-1-AP) by Proteintech is a Polyclonal antibody targeting DCK in WB, ELISA applications with reactivity to human samples 17758-1-AP targets DCK in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293T cells, HeLa cells, Jurkat cells, MCF-7 cells, HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:3000 Background Information Deoxycytidine kinase (dCK) is required for the phosphorylation of several deoxyribonucleoside analogues that are widely employed as chemotherapeutic agents(PMID:12628850). It is also a key enzyme in the phosphorylation of a variety of antineoplastic and antiviral nucleoside analogs including cytosine arabinoside (araC) and dideoxycytidine (ddCyd); deficiency of deoxycytidine kinase activity mediates resistance to these drugs(PMID:1996353). This protein can exsit as a homodimer(PMID:16421443). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12123 Product name: Recombinant human DCK protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-260 aa of BC103764 Sequence: MATPPKRSCPSFSASSEGTRIKKISIEGNIAAGKSTFVNILKQLCEDWEVVPEPVARWCNVQSTQDEFEELTMSQKNGGNVLQMMYEKPERWSFTFQTYACLSRIRAQLASLNGKLKDAEKPVLFFERSVYSDRYIFASNLYESECMNETEWTIYQDWHDWMNNQFGQSLELDGIIYLQATPETCLHRIYLRGRNEEQGIPLEYLEKLHYKHESWLLHRTLKTNFDYLQEVPILTLDVNEDFKDKYESLVEKVKEFLSTL Predict reactive species Full Name: deoxycytidine kinase Calculated Molecular Weight: 260 aa, 31 kDa Observed Molecular Weight: 30 kDa GenBank Accession Number: BC103764 Gene Symbol: DCK Gene ID (NCBI): 1633 RRID: AB_2261476 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P27707 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.