Proteintech, 17769-1-AP, BMP-9 Polyclonal antibody

Size: 20ul / 150ul The BMP-9 (17769-1-AP) by Proteintech is a Polyclonal antibody targeting BMP-9 in WB, IHC, ELISA applications with reactivity to human samples 17769-1-AP targets BMP-9 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: L02 cells, SMMC-7721 cells Positive IHC detected in: human liver cancer tissue, human hepatocirrhosis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information BMP9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein plays a role in the adult liver and in differentiation of cholinergic central nervous system neurons. Mutations in this gene are associated with hereditary hemorrhagic telangiectasia. Human BMP9 precursor protein contains 429 amino acids and has a calculated molecular mass of about 47 kD. Posttranslational processing results in a mature BMP9 protein of about 13 kD. Once secreted, BMP9 may exist as dimers of mature regions or as BMP9 proregion complexes in which the proregion of BMP9 remains tightly associated with the mature dimer. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12177 Product name: Recombinant human BMP9 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 23-429 aa of BC074921 Sequence: KPLQSWGRGSAGGNAHSPLGVPGGGLPEHTFNLKMFLENVKVDFLRSLNLSGVPSQDKTRVEPPQYMIDLYNRYTSDKSTTPASNIVRSFSMEDAISITATEDFPFQKHILLFNISIPRHEQITRAELRLYVSCQNHVDPSHDLKGSVVIYDVLDGTDAWDSATETKTFLVSQDIQDEGWETLEVSSAVKRWVRSDSTKSKNKLEVTVESHRKGCDTLDISVPPGSRNLPFFVVFSNDHSSGTKETRLELREMISHEQESVLKKLSKDGSTEAGESSHEEDTDGHVAAGSTLARRKRSAGAGSHCQKTSLRVNFEDIGWDSWIIAPKEYEAYECKGGCFFPLADDVTPTKHAIVQTLVHLKFPTKVGKACCVPTKLSPISVLYKDDMGVPTLKYHYEGMSVAECGCR Predict reactive species Full Name: growth differentiation factor 2 Calculated Molecular Weight: 429 aa, 47 kDa Observed Molecular Weight: 30 kDa, 46 kDa GenBank Accession Number: BC074921 Gene Symbol: BMP9 Gene ID (NCBI): 2658 RRID: AB_2878437 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UK05 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.