Proteintech, 17841-1-AP, MGAT4C Polyclonal antibody

Size: 20ul / 150ul The MGAT4C (17841-1-AP) by Proteintech is a Polyclonal antibody targeting MGAT4C in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 17841-1-AP targets MGAT4C in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, rat testis tissue Positive IP detected in: mouse testis tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information MGAT4C is predicted to be a glycosyltransferase that catalyzes a GlcNAc β1-4 linkage to the core mannose residues of N-glycans, resulting in N-glycans with tri- or tetraantennary structures.Recently MGAT4C has been found to get involved in processing complex N-glycan structures on CD133 and CD133 has been identified as a substrate of MGAT4C. Given to the fact that CD133 is widely used as a marker of numerous stem cell and cancer stem cell types, MGAT4C may have an important role in certain cancers. (PMID: 21937449) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12201 Product name: Recombinant human MGAT4C protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 127-478 aa of BC064141 Sequence: SSYEELKEISVVVHLADFNSSWRDAMVQDITQKFAHHIIAGRLMVIHAPEEYYPILDGLKRNYNDPEDRVKFRSKQNVDYAFLLNFCANTSDYYVMLEDDVRCSKNFLTAIKKVIASLEGTYWVTLEFSKLGYIGKLYHSHDLPRLAHFLLMFYQEMPCDWLLTHFRGLLAQKNVIRFKPSLFQHMGYYSSYKGTENKLKDDDFEEESFDIPDNPPASLYTNMNVFENYEASKAYSSVDEYFWGKPPSTGDVFVIVFENPIIIKKIKVNTGTEDRQNDILHHGALDVGENVMPSKQRRQCSSYLRLGEFKNGNFEMSGVNQKIPFDIHCMRIYVTKTQKEWLIIRSISIWTS Predict reactive species Full Name: mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme C (putative) Calculated Molecular Weight: 478 aa, 56 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC064141 Gene Symbol: MGAT4C Gene ID (NCBI): 25834 RRID: AB_2143048 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UBM8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.