Proteintech, 17889-1-AP, SLTM Polyclonal antibody

Size: 20ul / 150ul The SLTM (17889-1-AP) by Proteintech is a Polyclonal antibody targeting SLTM in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 17889-1-AP targets SLTM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, HeLa cells Positive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information SLTM, also named as Modulator of estrogen-induced transcription, is a 1034 amino acid protein, which localizes in the nucleus. When it is overexpressed, SLTM acts as a general inhibitor of transcription that eventually leads to apoptosis. The calculated molecular weight of SLTM is 117 kDa, but modified SLTM is about 135-140 kDa. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12060 Product name: Recombinant human SLTM protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 154-502 aa of BC046119 Sequence: RERIRIEQERRKEAERIAREREELRRQQQQLRYEQEKRNSLKRPRDVDHRRDDPYWSENKKLSLDTDARFGHGSDYSRQQNRFNDFDHRERGRFPESSAVQSSSFERRDRFVGQSEGKKARPTARREDPSFERYPKNFSDSRRNEPPPPRNELRESDRREVRGERDERRTVIIHDRPDITHPRHPREAGPNPSRPTSWKSEGSMSTDKRETRVERPERSGREVSGHSVRGAPPGNRSSASGYGSREGDRGVITDRGGGSQHYPEERHVVERHGRDTSGPRKEWHGPPSQGPSYHDTRRMGDGRAGAGMITQHSSNASPINRIVQISGNSMPRGSGSGFKPFKGGPPRRF Predict reactive species Full Name: SAFB-like, transcription modulator Calculated Molecular Weight: 478aa,57 kDa; 1034aa,117 kDa Observed Molecular Weight: 135-140 kDa GenBank Accession Number: BC046119 Gene Symbol: SLTM Gene ID (NCBI): 79811 RRID: AB_2192729 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NWH9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.