Proteintech, 18003-1-AP, DNA Polymerase Beta Polyclonal antibody

Size: 20ul / 150ul The DNA Polymerase Beta (18003-1-AP) by Proteintech is a Polyclonal antibody targeting DNA Polymerase Beta in WB, IHC, IP, ELISA applications with reactivity to human samples 18003-1-AP targets DNA Polymerase Beta in WB, IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: COLO 320 cells, K-562 cells Positive IP detected in: K-562 cells Positive IHC detected in: human testis tissue, human brain tissue, human kidney tissue, human ovary tissue, human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information DNA polymerase β (DNA polymerase beta (POLB)) belongs to a member of the DNA polymerase X family, mainly involved in various biological metabolic processes, such as eukaryotic DNA replication, DNA damage repair, gene recombination, and cell cycle regulation (PMID: 38731327). Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12496 Product name: Recombinant human POLB protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-335 aa of BC100288 Sequence: MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE Predict reactive species Full Name: polymerase (DNA directed), beta Calculated Molecular Weight: 335 aa, 38 kDa Observed Molecular Weight: 38 kDa GenBank Accession Number: BC100288 Gene Symbol: DNA Polymerase Beta Gene ID (NCBI): 5423 RRID: AB_2299845 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P06746 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.