Proteintech, 18029-1-AP, CARD6 Polyclonal antibody

Size: 20ul / 150ul The CARD6 (18029-1-AP) by Proteintech is a Polyclonal antibody targeting CARD6 in WB, IHC, ELISA applications with reactivity to human samples 18029-1-AP targets CARD6 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells Positive IHC detected in: human testis tissue, human kidney tissue, human lung tissue, human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information The caspase recruitment domain (CARD) is a homotypic protein-protein interaction module that links components of signal transduction pathways implicated in the regulation of apoptosis or adaptive or innate immunity. Although much progress has been made in assigning precise roles to most CARD-containing proteins, the functions of the 1,037-amino-acid (aa) human and 1,175-aa mouse CARD6 proteins are still unknown. CARD6 has a unique structure in that it contains the CARD at the N terminus, a glutamic acid-rich region following the CARD, and a proline-rich region at the C terminus. CARD6 also harbors a 350-aa region with similarity to upregulated gene 4 (URG4), a protein that is induced in response to hepatitis Bx antigen overexpression and exerts a positive effect on proliferation. Both CARD6 and URG4 share structural features with members of the multifaceted, IFN-inducible GTPase (IFNiGTPase) superfamily, which contains some of the proteins most abundantly induced during cell-autonomous immune responses. The calcualted molecular weight of CARD is 116 kDa, but modified CARD6 is about 130 kDa. (PMID: 18160713) Specification Tested Reactivity: human Cited Reactivity: rat, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12595 Product name: Recombinant human CARD6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC093825 Sequence: MATESTPSEIIERERKKLLEILQHDPDSILDTLTSRRLISEEEYETLENVTDLLKKSRKLLILVQKKGEATCQHFLKCLFSTFPQSAAICGLRHEVLKHENTVPPQSMGASSNSEDAFSPGIKQPEAPEITVFFSEKEHLDLETSEFFRDKKTSYRETALSARKNEKEYDTPEVTLSYSVEKVGCEVPATITYIKDGQRYEELDDSLYLGKEEYLGSVDTPEDAEATVEEEVYDDPEHVGYDGEEDFENSETTEFSGEEPSYEGSETSLSLEEEQEKSIEERKKVFKDVLLCLNMDRSRKVLPDFVKQFSLDRGCKWTPESPGDLAWNFLMKVQARDVTARDSILSHKVLD Predict reactive species Full Name: caspase recruitment domain family, member 6 Calculated Molecular Weight: 1037 aa, 116 kDa Observed Molecular Weight: 130 kDa GenBank Accession Number: BC093825 Gene Symbol: CARD6 Gene ID (NCBI): 84674 RRID: AB_2290686 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BX69 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.