Proteintech, 18032-1-AP, EDAR Polyclonal antibody

Size: 20ul / 150ul The EDAR (18032-1-AP) by Proteintech is a Polyclonal antibody targeting EDAR in WB, IP, ELISA applications with reactivity to human samples 18032-1-AP targets EDAR in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HEK-293 cells, A431 cells, MCF-7 cells Positive IP detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information EDAR, also named as DL, is a receptor for EDA isoform A1, but not for EDA isoform A2. It mediates the activation of NF-kappa-B and JNK. EDAR may promote caspase-independent cell death. It is expressed in adjacent or partially overlapping layers in the developing human skin with MW 48 kDa. Specification Tested Reactivity: human Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12601 Product name: Recombinant human EDAR protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 207-448 aa of BC093872 Sequence: MFYILKTKPSAPACCTSHPGKSVEAQVSKDEEKKEAPDNVVMFSEKDEFEKLTATPAKPTKSENDASSENEQLLSRSVDSDEEPAPDKQGSPELCLLSLVHLAREKSATSNKSAGIQSRRKKILDVYANVCGVVEGLSPTELPFDCLEKTSRMLSSTYNSEKAVVKTWRHLAESFGLKRDEIGGMTDGMQLFDRISTAGYSIPELLTKLVQIERLDAVESLCADILEWAGVVPPASQPHAAS Predict reactive species Full Name: ectodysplasin A receptor Calculated Molecular Weight: 448 aa, 49 kDa Observed Molecular Weight: 48 kDa GenBank Accession Number: BC093872 Gene Symbol: EDAR Gene ID (NCBI): 10913 RRID: AB_10596932 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UNE0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.