Proteintech, 18035-1-AP, LONP2 Polyclonal antibody

Size: 20ul / 150ul The LONP2 (18035-1-AP) by Proteintech is a Polyclonal antibody targeting LONP2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 18035-1-AP targets LONP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Jurkat cells Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:100-1:400 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information LONP2 (Lon Peptidase 2, Peroxisomal) is an important ATP-dependent protease. It is primarily located in peroxisomes and is responsible for maintaining the proteostasis of this organelle. By degrading misfolded proteins and selectively regulating metabolic enzymes, LONP2 plays a central role in maintaining peroxisomal proteostasis and metabolic balance. Dysfunction of LONP2 is closely related to a variety of human diseases, including peroxisomal disorders, cancer, and neurodegenerative diseases. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12650 Product name: Recombinant human LONP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 503-852 aa of BC093910 Sequence: DRMEIIQVPGYTQEEKIEIAHRHLIPKQLEQHGLTPQQIQIPQVTTLDIITRYTREAGVRSLDRKLGAICRAVAVKVAEGQHKEAKLDRSDVTEREGCREHILEDEKPESISDTTDLALPPEMPILIDFHALKDILGPPMYEMEVSQRLSQPGVAIGLAWTPLGGEIMFVEASRMDGEGQLTLTGQLGDVMKESAHLAISWLRSNAKKYQLTNAFGSFDLLDNTDIHLHFPAGAVTKDGPSAGVTIVTCLASLFSGRLVRSDVAMTGEITLRGLVLPVGGIKDKVLAAHRAGLKQVIIPRRNEKDLEGIPGNVRQDLSFVTASCLDEVLNAAFDGGFTVKTRPGLLNSKL Predict reactive species Full Name: lon peptidase 2, peroxisomal Calculated Molecular Weight: 852 aa, 95 kDa Observed Molecular Weight: 95 kDa GenBank Accession Number: BC093910 Gene Symbol: LONP2 Gene ID (NCBI): 83752 RRID: AB_2878485 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86WA8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.