Proteintech, 18052-1-AP, fetuin-B Polyclonal antibody

Size: 20ul / 150ul The fetuin-B (18052-1-AP) by Proteintech is a Polyclonal antibody targeting fetuin-B in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 18052-1-AP targets fetuin-B in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human blood tissue, human plasma Positive IP detected in: human plasma tissue Positive IHC detected in: human liver cancer tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12562 Product name: Recombinant human fetuin-B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 23-382 aa of BC074734 Sequence: QLALNPSALLSRGCNDSDVLAVAGFALRDINKDRKDGYVLRLNRVNDAQEYRRGGLGSLFYLTLDVLETDCHVLRKKAWQDCGMRIFFESVYGQCKAIFYMNNPSRVLYLAAYNCTLRPVSKKKIYMTCPDCPSSIPTDSSNHQVLEAATESLAKYKNENTSKQYSLFKVTRASSQWVVGPSYFVEYLIKESPCTKSQASSCSLQSSDSVPVGLCKGSLTRTHWEKFVSVTCDFFESQAPATGSENSAVNQKPTNLPKVEESQQKNTPPTDSPSKAGPRGSVQYLPDLDDKNSQEKGPQEAFPVHLDLTTNPQGETLDISFLFLEPMEEKLVVLPFPKEKARTAECPGPAQNASPLVLPP Predict reactive species Full Name: fetuin B Calculated Molecular Weight: 382 aa, 42 kDa Observed Molecular Weight: 50-60 kDa GenBank Accession Number: BC074734 Gene Symbol: Fetuin B Gene ID (NCBI): 26998 RRID: AB_2103181 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UGM5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.