Proteintech, 18139-1-AP, HYAL4 Polyclonal antibody

Size: 20ul / 150ul The HYAL4 (18139-1-AP) by Proteintech is a Polyclonal antibody targeting HYAL4 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 18139-1-AP targets HYAL4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: K-562 cells, NIH/3T3 cells, mouse testis tissue, rat testis tissue Positive IP detected in: mouse testis tissue Positive IHC detected in: mouse skin tissue, human placenta tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Hyaluronic acid (HA), a glycosaminoglycan that is ubiquitously present in the extracellular space of higher animals, maintains matrix structure and controls cellular functions such as proliferation, differentiation, and locomotion. Hyal enzymes that catabolize HA, are involved in development and tumorigenesis.HYAL4, a member of the Hyal family, is a chondroitin sulfate (CS)-specific endo-β-acetylgalactosaminidase. The expression of hHYAL4 mRNA is not ubiquitous but restricted to placenta, skeletal muscle, and testis (PMID: 23086929). This polyclonal antibody raised against 29-460aa of human HYAL4. The apparent molecular weight is larger than the calculated molecular weight of 54 kDa, which is likely due to posttranslational modification, presumably by glycosylation (PMID: 19889881). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12766 Product name: Recombinant human HYAL4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 29-460 aa of BC104788 Sequence: LKSISCLKPARLPIYQRKPFIAAWNAPTDQCLIKYNLRLNLKMFPVIGSPLAKARGQNVTIFYVNRLGYYPWYTSQGVPINGGLPQNISLQVHLEKADQDINYYIPAEDFSGLAVIDWEYWRPQWARNWNSKDVYRQKSRKLISDMGKNVSATDIEYLAKVTFEESAKAFMKETIKLGIKSRPKGLWGYYLYPDCHNYNVYAPNYSGSCPEDEVLRNNELSWLWNSSAALYPSIGVWKSLGDSENILRFSKFRVHESMRISTMTSHDYALPVFVYTRLGYRDEPLFFLSKQDLVSTIGESAALGAAGIVIWGDMNLTSSKANCTKVKQFVSSDLGSYIANVTRAAEVCSLHLCRNNGRCIRKMWNAPSYLHLNPASYHIEASEDGEFTVKGKASDTDLAVMADTFSCHCYQGYEGADCREIKTADGCSGVSP Predict reactive species Full Name: hyaluronoglucosaminidase 4 Calculated Molecular Weight: 481 aa, 54 kDa Observed Molecular Weight: 60-65 kDa GenBank Accession Number: BC104788 Gene Symbol: HYAL4 Gene ID (NCBI): 23553 RRID: AB_2878506 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q2M3T9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.