Proteintech, 18164-1-AP, PMS2 Polyclonal antibody

Size: 20ul / 150ul The PMS2 (18164-1-AP) by Proteintech is a Polyclonal antibody targeting PMS2 in WB, ELISA applications with reactivity to human, mouse, rat samples 18164-1-AP targets PMS2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, PC-3 cells, Jurkat cells, MCF-7 cells, Raji cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS). This antibody is specific to PMS2. The calculated molecular weight of PMS2 is 96 kDa, but the modified PMS2 is about 117 kDa.(PMID: 11358834) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12651 Product name: Recombinant human PMS2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 513-862 aa of BC093921 Sequence: SHCSSEYAASSPGDRGSQEHVDSQEKAPETDDSFSDVDCHSNQEDTGCKFRVLPQPTNLATPNTKRFKKEEILSSSDICQKLVNTQDMSASQVDVAVKINKKVVPLDFSISSLAKRIKQLHHEAQQSEGEQNYRKFRAKICPGENQAAEDELRKEISKTMFAEMEIIGQFNLGFIITKLNEDIFIVDQHATDEKYNFEMLQQHTVLQGQRLIAPQTLNLTAVNEAVLIENLEIFRKNGFDFVIDENAPVTERAKLISLPTSKNWTFGPQDVDELIFMLSDSPGVMCRPSRVKQMFASRACRKSVMIGTALNTSEMKKLITHMGEMDHPWNCPHGRPTMRHIANLGVISQN Predict reactive species Full Name: PMS2 postmeiotic segregation increased 2 (S. cerevisiae) Calculated Molecular Weight: 862 aa, 96 kDa Observed Molecular Weight: 117 kDa, 100 kDa GenBank Accession Number: BC093921 Gene Symbol: PMS2 Gene ID (NCBI): 5395 RRID: AB_2167145 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P54278 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.