Proteintech, 18216-1-AP, LSR Polyclonal antibody

Size: 20ul / 150ul The LSR (18216-1-AP) by Proteintech is a Polyclonal antibody targeting LSR in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 18216-1-AP targets LSR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HT-29 cells, HepG2 cells, mouse liver tissue, rat liver tissue Positive IHC detected in: mouse stomach tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information LSR (lipolysis stimulated lipoprotein receptor) is a lipoprotein receptor primarily expressed in the liver and activated by free fatty acids (PMID: 10224102). This remnant receptor binds both apoB- and apoE-containing lipoproteins and displays greatest affinity for TG-rich lipoproteins. Distribution of LSR causes embryonic lethality in mice, indicating that the expression of LSR is critical for liver and embryonic development (PMID: 15265030). Investigations show that LSR is a multimer consisting of α subunit (68 kDa) and β subunit (56 kDa) associated through disulfide bridges (PMID: 10224102; 19188430; 18644789). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12961 Product name: Recombinant human LSR protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 339-589 aa of BC004381 Sequence: SMRVLYYMEKELANFDPSRPGPPSGRVERAMSEVTSLHEDDWRSRPSRGPALTPIRDEEWGGHSPRSPRGWDQEPAREQAGGGWRARRPRARSVDALDDLTPPSTAESGSRSPTSNGGRRSRAYMPPRSRSRDDLYDQDDSRDFPRSRDPHYDDFRSRERPPADPRSHHHRTRDPRDNGSRSGDLPYDGRLLEEAVRKKGSEERRRPHKEEEEEAYYPPAPPPYSETDSQASRERRLKKNLALSRESLVV Predict reactive species Full Name: lipolysis stimulated lipoprotein receptor Calculated Molecular Weight: 71 kDa Observed Molecular Weight: 60-70 kDa, 56 kDa GenBank Accession Number: BC004381 Gene Symbol: LSR Gene ID (NCBI): 51599 RRID: AB_2250137 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86X29 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.