Proteintech, 18244-1-AP, EIF2AK2, PKR Polyclonal antibody

Size: 20ul / 150ul The EIF2AK2, PKR (18244-1-AP) by Proteintech is a Polyclonal antibody targeting EIF2AK2, PKR in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 18244-1-AP targets EIF2AK2, PKR in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, Jurkat cells, mouse thymus tissue, HepG2 cells, HeLa cells, rat heart tissue, K-562 cells, MCF-7 cells, SH-SY5Y cells, THP-1 cells Positive IP detected in: HeLa cells Positive IHC detected in: human kidney tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, pig, rabbit, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13010 Product name: Recombinant human EIF2AK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-373 aa of BC093676 Sequence: MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGT Predict reactive species Full Name: eukaryotic translation initiation factor 2-alpha kinase 2 Calculated Molecular Weight: 551 aa, 62 kDa Observed Molecular Weight: 65-68 kDa GenBank Accession Number: BC093676 Gene Symbol: EIF2AK2 Gene ID (NCBI): 5610 RRID: AB_2246451 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P19525 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.