Proteintech, 18299-1-AP, HER2/ErbB2 Polyclonal antibody

Size: 20ul / 150ul The HER2/ErbB2 (18299-1-AP) by Proteintech is a Polyclonal antibody targeting HER2/ErbB2 in WB, IHC, IF-Fro, IP, ELISA applications with reactivity to human, mouse samples 18299-1-AP targets HER2/ErbB2 in WB, IHC, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: 4T1 cells, SGC-7901 cells, MCF-7 cells, HeLa cells Positive IP detected in: SGC-7901 cells Positive IHC detected in: human breast cancer tissue, human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-Fro detected in: mouse breast cancer Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:300-1:1200 Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500 Background Information HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas. This antibody raised against a synthesized peptide corresponding to 1237-1255aa of human HER2 recognizes the 185-kDa full-length glycosylated form and other lower molecular-mass forms of HER2, including the truncated form. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) Calculated Molecular Weight: 138 kDa Observed Molecular Weight: 185 kDa GenBank Accession Number: BC167147 Gene Symbol: HER2/ErbB2 Gene ID (NCBI): 2064 RRID: AB_2099264 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P04626 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.