Proteintech, 18350-1-AP, DTX1 Polyclonal antibody

Size: 20ul / 150ul The DTX1 (18350-1-AP) by Proteintech is a Polyclonal antibody targeting DTX1 in WB, IHC, IF, ELISA applications with reactivity to human, mouse, rat samples 18350-1-AP targets DTX1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Daudi cells, A375 cells Positive IHC detected in: human colon tissue, human gliomas tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF detected in: mouse olfactory epithelium tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:150-1:600 Immunofluorescence (IF): IF : 1:50-1:200 Background Information Deltex homolog 1 (E3 ubiquitin-protein ligase DTX1) is a regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Functions as an ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.DTX1 is conserved from Drosophila, and is expressed in a wide array of tissues, particularly in the central nervous system, testes, and endothelial cells, mainly colocalized with Notch1.It contains a RING-type zinc finger and 2 WWE domains. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13172 Product name: Recombinant human DTX1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-374 aa of BC048216 Sequence: MSRPGHGGLMPVNGLGFPPQNVARVVVWEWLNEHSRWRPYTATVCHHIENVLKEDARGSVVLGQVDAQLVPYIIDLQSMHQFRQDTGTMRPVRRNFYDPSSAPGKGIVWEWENDGGAWTAYDMDICITIQNAYEKQHPWLDLSSLGFCYLIYFNSMSQMNRQTRRRRRLRRRLDLAYPLTVGSIPKSQSWPVGASSGQPCSCQQCLLVNSTRAASNAILASQRRKAPPAPPLPPPPPPGGPPGALAVRPSATFTGAALWAAPAAGPAEPAPPPGAPPRSPGAPGGARTPGQNNLNRPGPQRTTSVSARASIPPGVPALPVKNLNGTGPVHPALAGMTGILLCAAGLPVCLTRAPKPILHPPPVSKSDVKPVPGV Predict reactive species Full Name: deltex homolog 1 (Drosophila) Calculated Molecular Weight: 620 aa, 67 kDa Observed Molecular Weight: 67 kDa GenBank Accession Number: BC048216 Gene Symbol: DTX1 Gene ID (NCBI): 1840 RRID: AB_2878536 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86Y01 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.