Proteintech, 18469-1-AP, PIAS4 Polyclonal antibody

Size: 20ul / 150ul The PIAS4 (18469-1-AP) by Proteintech is a Polyclonal antibody targeting PIAS4 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 18469-1-AP targets PIAS4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HL-60 cells, Jurkat cells, K-562 cells Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information PIAS4(protein inhibitor of activated STAT protein 4) is also named as PIASG, PIASy and belongs to the PIAS family. It enhanced the conjugation of SUMO2 to GATA2 through its E3 SUMO ligase activity. TGF-b can up-regulates PLAS4 expression in MCs to down-regulatea-SMA gene transcription by the interaction with E12 and PLAS4 predominantly acts as E3 ligases for E12 in MCs among PIAS family members(PMID:22829926). This protein can be sumoylated and Lys-35 is the main site of sumoylation(PMID:15831457). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13316 Product name: Recombinant human PIAS4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 158-506 aa of BC010047 Sequence: SPCIFALTPRQVELIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQYPPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLVDGSWCPIRAEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGGGPVGSMENGKPGADVVDLTLDSSSSSEDEEEEEEEEEDEDEEGPRPKRRCPFQKGLVPAC Predict reactive species Full Name: protein inhibitor of activated STAT, 4 Calculated Molecular Weight: 57 kDa Observed Molecular Weight: 57-70 kDa GenBank Accession Number: BC010047 Gene Symbol: PIAS4 Gene ID (NCBI): 51588 RRID: AB_2164380 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N2W9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.